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Fig. 4. <t>p21,</t> <t>PAI-1,</t> <t>and</t> <t>IL-6</t> protein expression levels and representative western blot bands detected in untreated control cells (CTR) and treated with doxorubicin 50 nM (DOXO) in (A) MIA-PaCa-2, (B) PANC-1, (C) Hepa-RG and (D) HLF cells. All data represent the results of nine biologically-independent replicates, expressed as the mean SD. *P ≤0.003, **P ≤0.002, ***P ≤0.0002 and ****P ≤0.0001 indicate statistically significant differences by an unpaired t-test with Welch’s correction.
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Fig. 4. p21, PAI-1, and IL-6 protein expression levels and representative western blot bands detected in untreated control cells (CTR) and treated with doxorubicin 50 nM (DOXO) in (A) MIA-PaCa-2, (B) PANC-1, (C) Hepa-RG and (D) HLF cells. All data represent the results of nine biologically-independent replicates, expressed as the mean SD. *P ≤0.003, **P ≤0.002, ***P ≤0.0002 and ****P ≤0.0001 indicate statistically significant differences by an unpaired t-test with Welch’s correction.

Journal: FEBS open bio

Article Title: Delta-9 desaturase reduction in gastrointestinal cells induced to senescence by doxorubicin.

doi: 10.1002/2211-5463.13945

Figure Lengend Snippet: Fig. 4. p21, PAI-1, and IL-6 protein expression levels and representative western blot bands detected in untreated control cells (CTR) and treated with doxorubicin 50 nM (DOXO) in (A) MIA-PaCa-2, (B) PANC-1, (C) Hepa-RG and (D) HLF cells. All data represent the results of nine biologically-independent replicates, expressed as the mean SD. *P ≤0.003, **P ≤0.002, ***P ≤0.0002 and ****P ≤0.0001 indicate statistically significant differences by an unpaired t-test with Welch’s correction.

Article Snippet: Membranes were incubated with the following antibodies: D9 (SCD1; dilution 1:400; Cell Signaling Technology), FASN (dilution 1:500; Immunological Sciences), p21 (dilution 1:500; Cell Signaling), SASP Ab Sempler kit- 38 461 T (PAI-1; dilution 1:400; and IL-6; dilution 1:400; Cell Signaling Technology) and b-actin (dilution 1:1000; Cell Signaling Technology).

Techniques: Expressing, Western Blot, Control